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目的了解大肠埃希菌(ECO)对3类6种常用抗菌药物的耐药性以及外排泵acra/acrb基因在临床分离株中的存在,探讨acra/acrb与大肠埃希菌多药耐药性间的相关性。方法药物敏感试验采用美国临床实验室标准化委员会推荐的纸片扩散法(K-B法)进行6种抗菌药物的药物敏感性检测;PCR技术检测大肠埃希菌携带acra/acrb基因的情况。结果耐药模式中以多药耐药为主占52.11%,其表型以CTX+G+S+CIP+LEV为主,8株,占11.27%;acra/acrb阳性率在多耐药株与双耐药株、单耐药株、全敏感株比较差异均有统计学意义。结论大肠埃希菌对3类6种抗菌药物普遍耐药,并且存在明显的多药耐药和交叉耐药现象;外排泵基因acra/acrb是大肠埃希菌多药耐药的重要原因之一。
Objective To investigate the resistance of Escherichia coli (ECO) to 3 kinds of 6 kinds of commonly used antibiotics and the existence of efflux pump acra / acrb gene in clinical isolates. To investigate the relationship between acra / acrb and Escherichia coli multidrug resistance Relevance between sexes. Methods Drug susceptibility tests Six drug-susceptibility tests were performed using the disk diffusion method (K-B method) recommended by the American Committee for Clinical Laboratory Standards; the PCR assay was used to detect the acra / acrb gene in Escherichia coli. Results The majority of drug-resistant models were multi-drug resistant (52.11%). The phenotypes were CTX + G + S + CIP + LEV and 8 strains (11.27%). The positive rates of acra / Double-resistant strains, single-resistant strains, fully sensitive strains were statistically significant differences. Conclusions Escherichia coli is generally resistant to three kinds of six kinds of antibacterials, and has obvious multidrug resistance and cross resistance; efflux pump gene acra / acrb is an important cause of multidrug resistance of Escherichia coli one.