论文部分内容阅读
辅酶I(NAD+)在0.005moL/LTris 0.01moL/LNaCl溶液(pH7.0)中,于钴离子注入修饰碳纤维电极上出现一个S形的还原波,半波电位为-1.45V(vs.SCE)。峰电流与NAD+的浓度在2.38×10-5～4.76×10-4mol/L(r=0 9992)和4.76×10-4～1.78×10-3mol/L(r=0.9982)之间成线性关系,检出限为1.19×10-5mol/L,回收率在96.7%～103.4%之间。用线性扫描、循环伏安法研究了钴离子注入修饰碳纤维电极上NAD+的电化学行为。电极反应机理为:NAD++e NAD·;NAD·+e+H+ NADH;2NAD·→NAD2。另外,钴离子注入修饰碳纤维电极对NAD+具有电催化作用。
Coenzyme I (NAD +) showed a S-shaped reduction wave on the cobalt ion-implanted carbon fiber electrode in 0.005moL / L 0.01mol / L NaCl solution (pH 7.0) with a half-wave potential of -1.45V (vs.SCE) . The peak current was linear with the concentration of NAD + between 2.38 × 10-5 ~ 4.76 × 10-4 mol / L (r = 0 9992) and 4.76 × 10-4 ~ 1.78 × 10-3 mol / L (r = 0.9982) , The detection limit was 1.19 × 10-5mol / L, the recovery was between 96.7% ~ 103.4%. The electrochemical behavior of NAD + on cobalt ion implanted modified carbon fiber electrodes was investigated by linear sweep and cyclic voltammetry. Electrode reaction mechanism: NAD + + e NAD ·; NAD · + e + H + NADH; 2NAD · → NAD2. In addition, the cobalt ion-implanted modified carbon fiber electrode has an electrocatalytic effect on NAD +.