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目的:通过检测TNF-α、IL-6在单纯BPH与合并组织学炎症的BPH组织中表达的差异及两组BPH患者在临床指标上的异同,探讨炎症与BPH的发生发展的关系。方法:收集TURP术的90份BPH患者标本,用HE染色法将90份标本染色后分单纯组(A组)35份与合并炎症组(B组)55份。采用免疫组织化学方法检测TNF-α、IL-6在各标本中的表达情况,记录90例患者临床指标PSA、体积、年龄、尿流率等数据结果。全部患者均病理检查回报为BPH。结果:B组55例均为慢性炎症,构成比为0.61。与A组相比,B组患者前列腺体积较大,PSA水平也较高,差异有统计学意义(P<0.05);而两组患者在年龄和尿流率比较上无统计学意义(P!0.05)。与A组比较,B组TNF-α与IL-6的表达显著增高(P<0.05)。IL-6和TNF-α表达结果相关性分析:前列腺体积与患者年龄有相关性,差异有统计学意义(r=0.430,P<0.001),前列腺体积与炎症程度也有相关性,差异有统计学意义(r=0.610和r=0.609,P<0.001);患者的PSA水平与炎症程度有相关性,差异有统计学意义(r=0.572r=0.487,P<0.01),而患者PSA水平与年龄无相关性,差异无统计学意义(r=0.065,P!0.1);通过偏倚相关分析控制年龄因素的影响后,炎症严重水平与BPH体积仍有明显相关性。结论:大部分BPH患者合并有前列腺炎症,并且以慢性炎症为主。合并炎症的BPH患者PSA水平相对较高,前列腺体积相对较大,但是在尿流率和年龄上的差异无统计学意义。IL-6、TNF-α两种促炎性因子可能对BPH和PSA的分泌起着促进作用。
OBJECTIVE: To investigate the relationship between inflammation and the occurrence and development of BPH by detecting the difference of expression of TNF-α and IL-6 between BPH and BPH tissues with histological inflammation and the clinical features of BPH patients. Methods: Ninety BPH samples from patients undergoing TURP were collected. Totally 90 samples were stained with hematoxylin and eosin (HE) and divided into 35 groups (group A) and 55 groups (group B). Immunohistochemistry was used to detect the expression of TNF-α and IL-6 in each specimen. The clinical data of 90 patients with PSA, volume, age and urine flow rate were recorded. All patients were pathologically reported BPH. Results: All the 55 cases in group B were chronic inflammation with a ratio of 0.61. Compared with group A, group B had larger prostate volume and higher PSA level (P <0.05), while there was no significant difference between the two groups in age and urinary flow rate (P <0.05). 0.05). Compared with group A, the expression of TNF-α and IL-6 in group B was significantly increased (P <0.05). Correlation analysis between the expression of IL-6 and TNF-α: There was a significant correlation between the prostate volume and the patient’s age (r = 0.430, P <0.001). There was also a correlation between the volume of prostate and the degree of inflammation (R = 0.610 and r = 0.609, P <0.001). There was a significant correlation between the level of PSA and the degree of inflammation (r = 0.572r = 0.487, P <0.01) There was no significant difference between the two groups (r = 0.065, P! 0.1). After controlling for the influence of age by the bias correlation analysis, there was still a significant correlation between the severity of inflammation and the volume of BPH. Conclusions: Most BPH patients have prostatitis associated with chronic inflammation. BPH patients with inflammation of the relatively high level of PSA, prostate volume is relatively large, but in the urinary flow rate and age were not statistically significant. Both proinflammatory cytokines IL-6 and TNF-α may promote the secretion of BPH and PSA.