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目的探讨实验红鲫对~(137)Cs辐射的氧化应激响应和氧化应激生物标记物。方法实验红鲫分别以0 Gy、1.94 Gy、3.88 Gy、7.76 Gy、15.53 Gy~(137)Cs辐照,分别于24 h,48 h,72 h,96 h取出肝脏、性腺,按试剂盒方法检测SOD、GSH-PX活性,于7 d和14 d取出肝脏、肾脏、心脏、脑,按Western blot方法检测HSP70含量。结果 1.94 Gy至15.53 Gy~(137)Cs辐照能引起实验红鲫氧化应激,SOD、GSH-PX活性和HSP70含量发生改变。1.94 Gy~(137)Cs辐照诱导实验红鲫肝脏SOD活性升高,3.88 Gy、7.76 Gy、15.53 Gy~(137)Cs辐照诱导实验红鲫肝脏SOD活性下降,且随辐射剂量升高,其SOD活性逐渐降低。1.94 Gy以上~(137)Cs辐照诱导实验红鲫性腺SOD活性下降,并随辐射剂量升高和时间的延长而下降,且下降幅度较大。各辐照处理组实验红鲫肝脏和性腺的GSH-PX活性变化不大,表现出随辐射剂量升高则活性降低的变化趋势。~(137)Cs辐照处理后7 d和14 d,各辐照处理组实验红鲫肝脏、肾脏、心脏和脑的HSP70表达量均比对照组的高,且随着~(137)Cs辐照剂量的增加和时间的延长而升高,均存在着一定的“剂量-时间-效应”关系。结论实验红鲫性腺对~(137)Cs辐射的氧化应激响应较敏感,性腺SOD和肝脏或肾脏的HSP70可作为氧化应激标志物。
Objective To investigate the oxidative stress response and oxidative stress biomarkers of ~ (137) Cs irradiated by experimental red crucian carp. Methods The red crucian carp was irradiated with 0 Gy, 1.94 Gy, 3.88 Gy, 7.76 Gy and 15.53 Gy 137 Cs respectively. The liver and gonads were removed at 24 h, 48 h, 72 h and 96 h, respectively. The activities of SOD and GSH-PX were detected. The liver, kidney, heart and brain were removed on day 7 and day 14, and the content of HSP70 was detected by Western blot. Results 1.94 Gy to 15.53 Gy ~ (137) Cs irradiation could cause oxidative stress, SOD, GSH-PX activity and HSP70 content in red crucian carp. The activity of SOD in livers of red crucian carp increased with irradiation dose of 1.94 Gy ~ (137) Cs, and the activity of SOD in livers of red crucian carp decreased with doses of 3.88 Gy, 7.76 Gy and 15.53 Gy ~ (137) Cs. With the increase of radiation dose, Its SOD activity decreased gradually. The activity of SOD in the gonads of red crucian carp (Carassius auratus gibelio) decreased from 1.94 Gy to (137) Cs irradiation, and decreased with the increase of radiation dose and the prolongation of time. The activity of GSH-PX in the liver and gonads of experimental red crucian carp did not change much in each irradiation treatment group, showing a decreasing trend as the radiation dose increased. The expression of HSP70 in the liver, kidney, heart and brain of the irradiated red crucian carp was higher than that of the control at 7 d and 14 d after ~ (137) Cs irradiation, According to the increase of dose and prolongation of time, there is a certain “dose-time-effect” relationship. Conclusion The gonads of experimental red crucian carp are sensitive to the oxidative stress induced by 137 Cs radiation. SOD of gonads and HSP70 of liver or kidney can be used as oxidative stress markers.