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目的观察吲哚并[3,2-B]咔唑-6-甲醛[6-formylindolo(3,2-b)carbazole,FICZ]活化芳香烃受体(aryl hydrocarbon receptor,AhR)对C57BL/6小鼠肠急性缺血再灌注(I/R)后肠道屏障功能的影响。方法取C57BL/6小鼠18只,分成假手术组、I/R组和FICZ干预(I/R+FICZ)组,每组6只。小鼠肠I/R模型:肠系膜上动脉夹闭30 min,再灌注6 h。苏木精-伊红(HE)染色观察小肠组织形态学的变化。蛋白质印迹和免疫荧光检测观察肠黏膜AhR与ZO-1的表达与分布情况。结果与假手术组比较,I/R组肠黏膜明显肿胀,部分绒毛脱落、变粗,甚至倒伏、断裂,而I/R+FICZ组肠黏膜无明显肿胀,绒毛结构相对完整,形态接近正常;免疫荧光显示I/R后肠上皮表面ZO-1连续性明显破坏,而I/R+FICZ组肠上皮ZO-1连续性明显恢复。肠上皮蛋白定量分析显示:与假手术组比较,I/R组和I/R+FICZ组小鼠小肠上皮ZO-1表达分别下降50.7%和32.3%(P<0.05);I/R+FICZ组较I/R组小肠上皮ZO-1表达上调37.2%(P<0.05)。结论 FICZ预处理可有效缓解小鼠急性I/R对肠黏膜结构与功能的损伤,可能机制是上调ZO-1的表达。
Aim To observe the effect of aryl hydrocarbon receptor (AhR) on indole [3,2-B] carbazole-6-carboxaldehyde (FICZ) Effect of intestinal barrier function after acute intestinal ischemia / reperfusion (I / R) in rats. Methods Eighteen C57BL / 6 mice were divided into sham operation group, I / R group and FICZ intervention group (I / R + FICZ), with 6 rats in each group. Mice intestinal I / R model: superior mesenteric artery occlusion 30 min, reperfusion 6 h. Morphological changes of small intestine were observed with hematoxylin and eosin (HE) staining. Western blotting and immunofluorescence detection of intestinal mucosa AhR and ZO-1 expression and distribution. Results Compared with the sham operation group, the intestinal mucosa of I / R group was obviously swollen, part of the hair loss, thickening and even lodging and rupture. However, the intestinal mucosa of I / R + FICZ group had no obvious swelling and the villus structure was relatively intact and the morphology was close to normal. Immunofluorescence showed that the continuity of ZO-1 on the surface of I / R was obviously destroyed, while the continuity of ZO-1 in intestinal epithelium of I / R + FICZ group was obviously recovered. The intestinal epithelial protein quantitative analysis showed that the ZO-1 expression in intestinal epithelium of I / R group and I / R + FICZ group decreased by 50.7% and 32.3%, respectively (P <0.05) compared with sham operation group; I / R + FICZ The expression of ZO-1 in intestinal epithelium of I / R group was increased by 37.2% (P <0.05). Conclusions FICZ pretreatment can effectively relieve the injury of intestinal mucosal structure and function induced by acute I / R in mice. The possible mechanism is that the expression of ZO-1 is up-regulated.