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聚β羟基烷酸盐(PHA)以其较好的生物相容性、可生物降解性和再生性成为最有前途的生物高分子材料.本文基于甘油作为基质在饱食-饥饿模式下富集合成PHA的混培物,以乙酸、丙酸、丁酸、乳酸和葡萄糖作为基质考察混培物合成PHA的基质广谱性,结果表明乳酸和乙酸作为基质时PHA的产率系数较高;使用不同比例乙酸/丙酸混合基质时,PHA产量随着乙酸含量的增加而增大,乙酸/丙酸为3∶1时PHA产量最高.通过活性污泥同时贮存与生长模型模拟与线性回归两种方法证实,在单一基质或乙酸/丙酸混合基质情况下,PHA合成速率与OUR存在线性关系,因此,基于在线OUR测量数据可以实时估计PHA合成量.
Poly (β-hydroxyalkanoate) (PHA) is the most promising biopolymer material due to its good biocompatibility, biodegradability and reproducibility.Based on the enrichment of glycerol as substrate in the starvation-starvation mode The results showed that the yield coefficient of PHA was higher when lactic acid and acetic acid were used as the matrix. Using the mixture of PHA and acetic acid, propionic acid, butyric acid, lactic acid and glucose as substrate, With different ratios of acetic acid / propionic acid mixed matrix, the yield of PHA increased with the increase of acetic acid content, and PHA reached the highest when acetic acid / propionic acid was 3:1. Simultaneous storage and growth model of activated sludge by simulation and linear regression The method demonstrates that there is a linear relationship between the rate of PHA synthesis and OUR in the presence of a single matrix or mixed acetate / propionate matrix, and therefore, the amount of PHA synthesized can be estimated in real time based on online OUR measurements.