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一、原理: 用乙醚提取酸化尿液中粪卟啉和卟啉原。在乙醚层加碘-盐酸溶液使各种叶啉原氧化成粪卟啉并提取粪卟啉。用分光光度计测定粪卟啉在Soret谱带的最大吸收峰,并以港带波长两侧光密度去较正各种存在的杂质。二、试剂: 1.冰醋酸。 2.乙醚。 3.5％盐酸(约5g/100ml):120ml浓盐酸用蒸馏水稀释到一升。
First, the principle: Extraction of acid in urine with acetone fecal porphyrin and porphyrin. Iodine - hydrochloric acid solution was added to the ether layer to oxidize all kinds of protoporphyrins to coproporphyrin and extract coproporphyrin. The maximum absorption peak of the fecal porphyrin in the Soret band was measured with a spectrophotometer and the various existing impurities were corrected for the optical density at both sides of the wavelength of the band. Second, reagents: 1. Glacial acetic acid. 2. Ether. 3.5% Hydrochloric acid (approx. 5 g / 100 ml): 120 ml of concentrated hydrochloric acid is diluted to 1 liter with distilled water.