论文部分内容阅读
目的采用HPLC-ELSD测定多烯磷脂酰胆碱胶囊中溶血磷脂酰胆碱的含量。方法色谱柱为硅胶(250mm×4.6mm,5μm),流动相A为正己烷-异丙醇(3∶4),流动相B为正己烷-异丙醇-水(3∶4∶0.75),梯度洗脱,流速为1.5mL·min-1,空气流速为3.0L·min-1,蒸发管温度为100℃。结果溶血磷脂酰胆碱0.1～1.5mg·mL-1与峰面积呈良好的线性关系(r=0.9997),平均回收率为99.90%。结论所用方法简便、专属性好,可用于多烯磷脂酰胆碱胶囊中溶血磷脂酰胆碱的含量检测。
Objective To determine the content of lysophosphatidylcholine in polyene phosphatidylcholine capsules by HPLC-ELSD. Methods The chromatographic column was silica gel (250 mm × 4.6 mm, 5 μm). The mobile phase A was n-hexane-isopropanol (3: 4), the mobile phase B was n-hexane- Gradient elution, the flow rate of 1.5mL · min-1, the air flow rate of 3.0L · min-1, the evaporation tube temperature of 100 ℃. Results The lysophosphatidylcholine had a good linear relationship (r = 0.9997) with the peak area of 0.1-1.5 mg · mL-1 and the average recovery was 99.90%. Conclusion The method is simple and specific, and can be used for the determination of lysophosphatidylcholine in polyene phosphatidylcholine capsules.