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目的：进一步探讨亚硝胺通过脂质过氧化而致癌的可能途径，以及研究β－胡萝卜素（β－Ｃ）和核黄素（ＶＢ２）通过抑制脂质过氧化反应而影响亚硝胺致癌的可能途径。方法：将５０只二级ＳＤ雄性大鼠随机分成５组。实验组在每日灌胃二甲基亚硝胺（ＮＤＭＮ）１．７５ｍｇ／ｋｇ的基础上，每日分别给予β－Ｃ２５ｍｇ／ｋｇ和／或ＶＢ２１８ｍｇ／ｋｇ补充，实验期为２８天。实验结束时，收集血液，摘取肝脏和肾脏，测红细胞和肝肾匀浆ＳＯＤ活性，全血ＧＳＨ－Ｐｘ活性，血清和肝肾匀浆ＭＤＡ含量，血清ＲＯＯＨ以及全血Ｈｂ含量。结果：１．ＮＤＭＮ可使ＳＯＤ和ＧＳＨ－Ｐｘ活性下降（Ｐ＜０．０５），ＭＤＡ和ＲＯＯＨ产生增多（Ｐ＜０．０５）。２．添加β－Ｃ后，ＳＯＤ和ＧＳＨ－Ｐｘ活性有明显提高（Ｐ＜０．０５），ＭＤＡ和ＲＯＯＨ的含量明显降低（Ｐ＜０．０５）。３．添加ＶＢ２后，肝ＳＯＤ和全血ＧＳＨ－Ｐｘ活性均有明显提高（Ｐ＜０．０５），但ＭＤＡ和ＲＯＯＨ的含量没有差异（Ｐ＞０．０５）。４．同时补充β－Ｃ和ＶＢ２后，各项指标的变化与单纯β－Ｃ组基本一致。结论：脂质过氧化也是亚硝胺致癌的途径之一；一定剂量的β－Ｃ可对抗ＮＤＭＮ引发的脂质过氧化；核黄素对提高抗氧化?
OBJECTIVE: To further explore the possible pathways by which nitrosamines cause cancer through lipid peroxidation and to investigate the effects of β-carotene (β-C) and riboflavin (VB2) on the carcinogenesis of nitrosamines by inhibiting lipid peroxidation Possible way. Methods: Fifty secondary SD male rats were randomly divided into five groups. The experimental group was given β-C25mg / kg and / or VB218mg / kg supplementation daily for 28 days on the basis of daily oral administration of dimethylnitrosamine (NDMN) 1.75mg / kg. At the end of the experiment, the blood was harvested, the liver and kidneys were harvested, the SOD activities of erythrocytes and homogenates of liver and kidney, the activities of GSH-Px in whole blood, the content of MDA in serum and liver homogenate, the content of serum ROOH and the content of Hb in whole blood were measured. Results: 1. NDMN could decrease the activities of SOD and GSH-Px (P <0.05) and increase the production of MDA and ROOH (P <0.05). 2. After adding β-C, the activities of SOD and GSH-Px were significantly increased (P <0.05), and the contents of MDA and ROOH were significantly decreased (P <0.05). 3. After adding VB2, the activities of hepatic SOD and whole blood GSH-Px were significantly increased (P <0.05), but the contents of MDA and ROOH were not different (P> 0.05). 4. After the addition of β-C and VB2, the change of each index was basically the same as that of the simple β-C group. Conclusion: Lipid peroxidation is also one of the ways of carcinogenesis of nitrosamine; a certain dose of β-C can be against lipid peroxidation induced by NDMN; riboflavin to improve anti-oxidation?