论文部分内容阅读
可鉴定人T淋巴细胞辅助/诱导(OKT_4)和细胞毒性/抑制(OKT_3)亚群的单克隆抗体,近年来不仅被用于研究人类疾病的免疫调节,而且被用于研究细胞群的免疫调节。但对免疫缺陷病患者的血液中OKT_4和OKT_3阳性淋巴细胞二者的比例和体外细胞免疫功能的研究尚少。作者检查了60名不同程度免疫缺陷病患者和20名健康人周围血液中单个核细胞对同种异体细胞(MLC)和PHA的增殖反应。同时应用间接免疫荧光法检查OKT_4和OKT_3淋巴细胞亚群。检查结果:正常对照组OKT_4阳性淋巴细胞范围为42—65%,百分率为56.0±5.3%(平均±1SD);OKT_3阳性淋巴细胞范围为15—42%,百分率为28.6±5.9%;
In recent years, monoclonal antibodies that can identify human T lymphocyte helper / inducer (OKT_4) and cytotoxic / inhibitory (OKT_3) subpopulations have been used not only to study immune regulation of human diseases but also to study immunomodulation of cell populations. However, there is little research on the ratio of OKT_4 and OKT_3-positive lymphocytes in the blood of immunodeficiency patients and in vitro cellular immune function. The authors examined the proliferative response of allogeneic cells (MLC) and PHA to peripheral blood mononuclear cells from 60 patients with immunodeficiency and 60 normal controls. OKT_4 and OKT_3 lymphocyte subsets were detected by indirect immunofluorescence. The results showed that the percentage of OKT_4 positive lymphocytes in normal control group was 42-65%, the percentage was 56.0 ± 5.3% (average ± 1SD); the OKT_3 positive lymphocyte range was 15-42%, the percentage was 28.6 ± 5.9%;