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目的 探讨基质金属蛋白酶２（ＭＭＰ２） ｍＲＮＡ 及蛋白在胃癌中的表达。方法 用ＲＴＰＣＲ 法及免疫组化法分别检测２５ 例胃癌患者癌组织及癌旁２ｃｍ 与≥５ｃｍ 组织ＭＭＰ２ ｍＲＮＡ 及蛋白的表达。结果 胃癌组织ＭＭＰ２ ｍＲＮＡ 表达率（１７／２５） 高于两组癌旁组织，与癌旁≥５ｃｍ 组织表达率（９／２５）比较差异有显著性（Ｐ＜０ ．０５）；免疫组化显示胃癌组织ＭＭＰ２ 蛋白的表达率（１７／２５） 也高于两组癌旁组织，与癌旁≥５ｃｍ 组织表达率（８／２５） 比较差异有显著性（Ｐ＜ ０ ．０５） 。结果接近ＲＴＰＣＲ 法；胃癌组织ＭＭＰ２ ｍＲＮＡ 表达值显著高于两组癌旁组织（Ｐ均＜０ ．０１）；ＭＭＰ２ ｍＲＮＡ 及蛋白表达似与胃癌分化及临床分期有关。１９ 例进展期胃癌组织及癌旁２ｃｍ 组织，ＭＭＰ２ ｍＲＮＡ 表达值显著高于６ 例早期胃癌相应组织（Ｐ均＜ ０．０５） 。结论 ＭＭＰ２ 在胃癌侵袭及转移过程中可能发挥重要作用。
Objective To investigate the expression of matrix metalloproteinase-2 (MMP-2) mRNA and protein in gastric cancer. Methods RTPCR and immunohistochemistry were used to detect the expression of MMP2 mRNA and protein in cancerous tissues and adjacent tissues 2cm and ≥5cm in 25 cases of gastric cancer. Results The expression rate of MMP 2 mRNA in gastric cancer tissue was higher than that in the adjacent tissues of the two groups (P<0.05). The difference was statistically significant (P<0.05). The expression rate of MMP 2 protein in gastric cancer tissue (17/25) was also higher than that in the adjacent tissues of the two groups, and there was a significant difference from the tissue expression rate (8/25) of ≥ 5cm adjacent to the tumor (P < 0.05). . The results were close to the RTPCR method; the expression of MMP2 mRNA in gastric cancer tissue was significantly higher than that in adjacent tissues (P<0.01); the expression of MMP2 mRNA and protein seemed to be related to the differentiation and clinical staging of gastric cancer. In 19 cases of advanced gastric cancer tissues and 2 cm of adjacent tissue, the expression of MMP 2 mRNA was significantly higher than that in 6 cases of early gastric cancer (P < 0.05). Conclusion MMP 2 may play an important role in the invasion and metastasis of gastric cancer.