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目的：研究金丝桃苷（Ｈｙｐ）对新生大鼠的脑细胞内游离钙浓度的作用．方法：分离新生大鼠的脑细胞；用钙离子荧光指示剂Ｆｕｒａ２测定静息和激动剂存在时新生鼠脑细胞内游离钙浓度．结果：在ＣａＣｌ２１３ｍｍｏｌ·Ｌ－１的Ｈａｎｋｓ液中，静息［Ｃａ２＋］ｉ为（２０８±１２）ｎｍｏｌ·Ｌ－１（ｎ＝１７），Ｈｙｐ对静息［Ｃａ２＋］ｉ无明显影响；Ｈｙｐ１０，４０，１６０μｍｏｌ·Ｌ－１呈浓度依赖性显著抑制ＫＣｌ５０ｍｍｏｌ·Ｌ－１致［Ｃａ２＋］ｉ增高；Ｈｙｐ１６０μｍｏｌ·Ｌ－１显著抑制去甲肾上腺素１，２，４和８μｍｏｌ·Ｌ－１诱发的［Ｃａ２＋］ｉ的增高；Ｈｙｐ１６０μｍｏｌ·Ｌ－１还可显著抑制谷氨酸和５羟色胺致［Ｃａ２＋］ｉ增高．结论：Ｈｙｐ对新生大鼠脑细胞钙内流有阻滞作用．
Objective: To study the effect of hyperin on intracellular free calcium concentration in neonatal rats. Methods: The brain cells of neonatal rats were isolated. Fura 2 was used to measure the intracellular free calcium concentration of neonatal rats in the presence of resting and agonist. Results: The resting [Ca2 +] i was (208 ± 12) nmol·L-1 (n = 17) in Hanks’ solution with 21.3 mmol·L-1 CaCl2 and Hyp had no effect on resting [Ca2 +] i ; Hyp10,40,160μmol·L-1 in a concentration-dependent manner significantly inhibited KCl50mmol·L-1-induced [Ca2 +] i increased; Hyp16 0μmol·L-1 significantly inhibited norepinephrine 1,2 , 4 and 8μmol·L-1 induced [Ca2 +] i increase; Hyp16 0μmol·L-1 also significantly inhibited glutamate and serotonin caused [Ca2 +] i increased. Conclusion: Hyp blocks the calcium influx in brain cells of neonatal rats.