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背景:炎性反应在动脉粥样硬化疾病的发生与发展,以及冠状动脉介入治疗后再狭窄的过程中起重要作用,但具体机制尚未明确。目的:观察兔腹主动脉球囊扩张后炎性因子白细胞介素8、白细胞介素6、肿瘤坏死因子α以及C-反应蛋白不同时间点的水平变化。设计、时间及地点:随机对照动物实验,于2008-01/08在河北省人民医院心脏中心导管室及临床研究中心完成。材料:选取8～12月龄新西兰大白兔24只,雌雄不拘,体质量2.50～3.25kg。方法:24只新西兰大白兔,随机抽签法分为实验组和对照组,每组12只。实验组穿刺股动脉后留置鞘管,经鞘管送入球囊行球囊扩张,拉伤腹主动脉。对照组仅穿刺留鞘,不进行球囊拉伤。分别于实验前、实验后4h,1,3d,1,2周,耳缘静脉采血4mL,3000r/min离心20min后,取上清液得血清标本,-80℃冰箱统一保存。主要观察指标:①酶联免疫吸附法分别测定实验前后不同时间点白细胞介素8、白细胞介素6、肿瘤坏死因子α和C-反应蛋白水平。②白细胞介素8水平与其他3种因子的相关性分析。③造影观察实验动物腹主动脉球囊损伤前后的形态学改变。结果:24只新西兰大白兔均进入结果分析。①兔腹主动脉球囊损伤后炎性因子白细胞介素8、白细胞介素6、肿瘤坏死因子α以及C-反应蛋白水平明显升高,与术前及对照组相比,差异具有显著性意义(P<0.01)。②各种炎性因子中,白细胞介素8水平最先升高,达峰值时间最短,相关分析显示,白细胞介素8水平表达与炎性反应标志物C-反应蛋白以及白细胞介素6、肿瘤坏死因子α呈正相关,相关系数r分别为0.569,0.673,0.597(P均<0.05)。③球囊扩张拉伤后2周,实验组动物腹主动脉拉伤处较对照组管腔明显狭窄。结论:兔腹主动脉球囊扩张后引起炎性因子白细胞介素8、白细胞介素6、肿瘤坏死因子α以及C-反应蛋白水平升高,导致动脉管腔狭窄,其中白细胞介素8在此过程中发挥了重要作用。
BACKGROUND: Inflammatory reactions play an important role in the development and progression of atherosclerotic disease and in the process of restenosis after coronary intervention. However, the exact mechanism is still unclear. OBJECTIVE: To observe the changes of inflammatory factors interleukin-8, interleukin-6, tumor necrosis factor-α and C-reactive protein at different time points after balloon dilation in rabbit abdominal aorta. DESIGN, TIME AND SETTING: A randomized controlled animal experiment was performed at the Cardiovascular Center and Clinical Research Center of Hebei Provincial People’s Hospital from January 2008 to December 2008. MATERIALS: Twenty-four New Zealand white rabbits (8-12 months old) were selected, both male and female, weighing 2.50-3.25kg. Methods: Twenty-four New Zealand white rabbits were randomly divided into experimental group and control group, with 12 in each group. The experimental group punctured femoral artery after leaving the sheath, the balloon into the balloon catheter balloon dilation, abdominal aorta injury. The control group only puncture the sheath, without balloon injury. Blood samples of 4mL, 1,3d, 1,2weeks after the experiment, 4mL of the ear vein were centrifuged at 3000r / min for 20min. The serum samples were collected at -80 ℃ and stored at -80 ℃. MAIN OUTCOME MEASURES: ① The levels of interleukin-8, interleukin-6, tumor necrosis factor-α and C-reactive protein were measured by enzyme linked immunosorbent assay before and after the experiment. ② Interleukin-8 levels and other three factors in the correlation analysis. ③ angiography observed experimental animals before and after abdominal aortic balloon injury morphological changes. Results: 24 New Zealand white rabbits were involved in the result analysis. ① The levels of interleukin-8, interleukin-6, tumor necrosis factor-α and C-reactive protein were significantly increased after balloon injury in abdominal aorta, which were significantly different from preoperative and control groups (P <0.01). ② Among various inflammatory factors, the level of interleukin-8 (IL-8) first increased and peaked at the shortest time. Correlation analysis showed that interleukin-8 level was correlated with C-reactive protein and interleukin-6 The correlation coefficient r were 0.569, 0.673 and 0.597, respectively (all P <0.05). ③ 2 weeks after balloon dilatation, the abdominal aorta in the experimental group was significantly stenosed than the control group. CONCLUSION: After the abdominal aorta balloon is inflated, the inflammatory cytokines interleukin-8, interleukin-6, tumor necrosis factor-alpha and C-reactive protein are increased, leading to stenosis of arterial lumen, of which interleukin-8 The process played an important role.