论文部分内容阅读
目的本研究评价和优化基于阳离子膜滤芯的环境水体病毒浓缩方法,为监测环境水体中的病毒提供方法依据。方法采用基于阳离子膜滤芯Nano Ceram的膜吸附洗脱法作为初级浓缩手段,结合有机絮凝和超滤法,从大体积初始水量中浓缩目标病毒。预实验评价不同有机絮凝条件对病毒基因拷贝数回收和病毒滴度的影响。结果随着有机絮凝牛肉浸膏洗脱液p H值的上升,回收病毒的基因拷贝数存在下降趋势(β=41.59,P<0.01)。采用2种浓缩流程回收4种水体中的目标病毒,成组t检验显示两者差异有统计学意义(t=2.261,P<0.05)。当调节有机絮凝洗脱液p H值为2.0时,水源水中脊髓灰质炎病毒的回收率最高达到7.87%。结论本研究建立的方法可作为今后环境水体常规监测的有效手段。
Objective This study evaluated and optimized the method of concentration of virus based on cationic membrane filter in environmental water body to provide a method basis for monitoring the virus in environmental water body. Methods A membrane-based adsorption and elution method based on Nano Ceram membrane was used as the primary concentration method. Organic flocculation and ultrafiltration were used to concentrate the target virus from a large volume of initial water. Pre-experimental evaluation of different organic flocculation conditions on viral gene copy number recovery and virus titer. Results As the p H value of organic flocculant beef extract increased, the gene copy number of recovered virus showed a decreasing trend (β = 41.59, P <0.01). Two kinds of concentration processes were used to recover the target virus in four kinds of water. The t-test showed that the difference between the two groups was statistically significant (t = 2.261, P <0.05). When the pH value of organic flocculant eluent was adjusted to 2.0, the recovery rate of poliovirus in water was up to 7.87%. Conclusion The method established in this study can be used as an effective means of routine monitoring of environmental water bodies in the future.