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近年用细胞化学、酶染色鉴别慢粒原始细胞危象时的细胞类型,POX(+)被认为是髓细胞特征,POX(-),Tdt(+)被认为淋巴样,两者均(-)为未分化型。本文作者分析30例Ph~1(+)慢粒淋巴样及未分化型原始细胞危象患者,治疗前经血、骨髓细胞化学(包括POX、PAS、ANAE、氯化醋酸)、Tdt酶染色及核型研究。用细胞流式仪分离原始细胞,以异硫氰酸盐(FITC)结合相应单克隆抗体;第2步用羊抗兔IgG对T细胞、B细胞及粒、单核细胞表面标记进行分型。显示20例为淋巴样,10例为未分化型原始细胞危象,慢性期中位数为24个月。Ph核型异常包括双倍Ph染色体、三体8、等臂染色体17、单体7或其它改变者14例(47%)。20例淋巴样原始细胞危象患者Calla(+)12例(60%),未分化型者无一例Calla(+)。电镜下进行
In recent years, cytokines and enzyme staining were used to identify the type of cells in the CML crisis. POX (+) was considered as a characteristic myeloid. POX (-) and Tdt (+) were considered lymphoid, Undifferentiated. The authors analyzed 30 Ph ~ 1 (+) chronic lymphocytic and undifferentiated blast crisis in patients with pre-treatment blood, bone marrow cytochemistry (including POX, PAS, ANAE, chlorinated acetic acid), Tdt staining and nuclear Type research. The original cells were separated by flow cytometer and the corresponding monoclonal antibodies were bound with isothiocyanate (FITC). Step 2 T-cell, B cell, and monocyte surface markers were sorted by goat anti-rabbit IgG. 20 cases showed lymphoid, 10 cases of undifferentiated primitive cell crisis, the median chronic phase was 24 months. Ph karyotype abnormalities include double Ph chromosome, trisomy 8, ischromosome 17, monomeric 7 or other altered in 14 cases (47%). Calla (+) was found in 12 cases (60%) of 20 cases of lymphoid protoco2 crisis, and none of cases of undifferentiated Calla (+). Electron microscopy