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[目的 ]探讨 β 胡萝卜素在吸烟诱导DNA损伤中的作用机制。[方法 ]人胚肺二倍体细胞SL 7单细胞电泳分析 (彗星试验 ) β 胡萝卜素和吸烟水溶性物质 (CSS)单独及联合作用 ,结合抗氧化剂VitE干预试验。[结果 ] 0 5～ 10μmol/Lβ 胡萝卜素单独作用不产生DNA断裂。 1∶10和 1∶2 0稀释CSS诱导彗星形成率分别达到 84%和 68% ,0 5和 1 0μmol/Lβ 胡萝卜素保护CSS对DNA损伤作用具有显著意义 ,但当 β 胡萝卜素浓度高 10倍时 ,则失去保护作用。 5 0 μmol/LVitE能有效抑制氧化损伤。 [结论 ]CSS可以诱导人胚肺二倍体细胞DNA损伤 ;β 胡萝卜素具有保护CSS诱导细胞DNA损伤作用 ,但当剂量升高至 5 0～ 10 0 μmol/L时 ,则失去保护作用 ,这可能与高浓度 β 胡萝卜素原氧化作用有关
[Objective] To explore the mechanism of β-carotene in smoking-induced DNA damage. [Method] Human embryo lung diploid cell SL 7 single cell electrophoresis analysis (comet assay) β-carotene and smoking water-soluble substance (CSS) alone and in combination with antioxidant VitE intervention test. [Result] 0 5 ~ 10μmol / L β-carotene alone did not produce DNA breaks. The CSS-induced rate of comet formation at 1:10 and 1:20 was 84% and 68%, respectively. CSS at 0 5 and 10 μmol / L protected CSS with significant effect on DNA damage. However, when β-carotene concentration was 10 times higher When, then the loss of protection. 5 0 μmol / LVitE can effectively inhibit oxidative damage. [Conclusion] CSS can induce DNA damage in human embryo lung diploid cells. Β-carotene has the protective effect of CSS on DNA damage induced by CSS. However, when the dosage is increased to 50 ~ 100 μmol / L, β-carotene loses its protective effect. It may be related to the high concentration of β-carotene protoxide