论文部分内容阅读
目的通过对宫颈病变中脆性组氨酸三联体(Fragile histidine traid,FHIT)基因上三个微卫星位点D3S1300、D3S1234和D3S1481的微卫星不稳定性(Microsatellite instability,MSI)分析,探讨其与宫颈癌的发生、发展的关系。方法采用聚合酶链反应-非变性聚丙烯酰胺凝胶电泳-溴化乙锭染色技术检测FHIT基因中3个微卫星位点D3S1300、D3S1234和D3S1481的MSI。结果 MSI在宫颈上皮内瘤样病变(Cervical intraepithelial neoplasia,CIN)组与宫颈癌组检出率差异有统计学意义。在宫颈癌中I期与II期差异无统计学意义,与III~IV期比较差异有统计学意义,MSI的发生率与临床分期有相关,与病理分级无关。结论 MSI在宫颈癌中的检出率远远高于CIN,表明MSI在宫颈癌发展过程中起重要作用,是宫颈癌恶性进展中的早期事件。
Objective To study the microsatellite instability (MSI) analysis of three microsatellite loci D3S1300, D3S1234 and D3S1481 in the Fragile histidine traid (FHIT) gene in cervical lesions, The occurrence and development of cancer. Methods The MSI of D3S1300, D3S1234 and D3S1481 at three microsatellite loci in FHIT gene was detected by polymerase chain reaction-non-denaturing polyacrylamide gel electrophoresis-ethidium bromide staining. Results The detection rate of MSI in Cervical intraepithelial neoplasia (CIN) group and cervical cancer group was significantly different. There was no significant difference between stage I and stage II in cervical cancer, and there was significant difference between stage I and IV. The incidence of MSI was correlated with clinical stage, but not with pathological grade. Conclusion The detection rate of MSI in cervical cancer is much higher than that in CIN, indicating that MSI plays an important role in the development of cervical cancer and is an early event in the malignant progression of cervical cancer.