论文部分内容阅读
目的:探讨survivin基因和NET-1蛋白在膀胱癌中的表达及其与临床病理特征的关系。方法:采用逆转录聚合酶链反应和免疫组化方法分别检测27例膀胱癌组织和10例正常膀胱组织survivin基因和NET-1蛋白的表达,并将结果进行相关分析。结果:27例膀胱癌组织中survivin基因阳性17例(62.96%),NET-1蛋白阳性21例(77.78%);10例正常膀胱组织未见survivin基因表达,NET-1蛋白表达10.00%(1/10),二者差异有统计学意义(P<0.01);survivin基因与NET-1蛋白表达有相关性(χ2=7.09,P<0.01),survivin基因和NET-1蛋白表达与膀胱癌WHO分级和TNM分期有关,与患者年龄和性别无关。结论:survivin基因和NET-1蛋白在膀胱癌中过度表达表明膀胱癌发生、发展与细胞凋亡和细胞增殖有关,survivin基因对膀胱癌早期诊断和靶向治疗有重要意义。
Objective: To investigate the expression of survivin and NET-1 in bladder cancer and its relationship with clinicopathological features. Methods: The expressions of survivin and NET-1 protein in 27 cases of bladder cancer and 10 cases of normal bladder tissues were detected by RT-PCR and immunohistochemistry. The results were analyzed. Results: Survivin gene was positive in 17 cases (62.96%) and NET-1 protein in 21 cases (77.78%) in 27 cases of bladder cancer. No survivin gene expression was found in 10 cases of normal bladder tissue, while NET-1 protein expression was 10.00% / 10). There was a significant difference between the two groups (P <0.01). Survivin was correlated with NET-1 protein expression (χ2 = 7.09, P <0.01) Grading is related to TNM staging, regardless of patient age and gender. CONCLUSIONS: The overexpression of survivin and NET-1 in bladder cancer indicates that bladder cancer is the most common cause of bladder cancer. The development of bladder cancer is related to apoptosis and cell proliferation. Survivin gene is of great importance for early diagnosis and targeted therapy of bladder cancer.