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胰岛素能影响卵巢功能,例如,离体颗粒细胞持续生长需要胰岛素,胰岛素也促进LH诱导的黄体细胞生成孕酮等。除猪颗粒细胞的资料外,人类卵巢组织也有胰岛素受体。Jarrett等在剖腹手术时取得人类卵巢组织样品,作质膜受体测定。质膜与猪[~(125)Ⅰ]胰岛素共同保温,Scatchard分析表明存在双相曲线。高亲和力结合位平均浓度为57.4±7.9(±SEM)fmol/mg蛋白质,解离常数为3.5±0.9nM。在月经周期中结合亲和力和结合位点数目皆无明显变化。上皮生长因子或高血糖素都不影响胰岛素与卵巢质膜的结合。因此,人类卵巢质膜存在高亲和力、特异性和可饱和的胰岛素结合位。胰岛素可能直接参与卵巢功能。
Insulin can affect ovarian function, for example, the sustained growth of isolated granular cells need insulin, insulin also promote LH-induced progesterone progesterone and so on. In addition to porcine granulosa cells, human ovarian tissue also has insulin receptors. Jarrett et al. Obtained human ovarian tissue samples during laparotomy for plasma membrane receptor assay. The plasma membrane was co-incubated with porcine [~ (125) I] insulin, and Scatchard analysis showed a biphasic curve. The average high-affinity binding site concentration was 57.4 ± 7.9 (± SEM) fmol / mg protein with a dissociation constant of 3.5 ± 0.9 nM. There was no significant change in binding affinity and number of binding sites during the menstrual cycle. Epidermal growth factor or glucagon does not affect insulin and the ovarian plasma membrane binding. Therefore, human ovarian plasma membrane exists high affinity, specificity and saturable insulin binding sites. Insulin may be directly involved in ovarian function.