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用荧光素标记的簇毛麦(Haynaldia villosa)基因组总DNA作探针,以普通小麦基因组总DNA作封阻,与花粉母细胞减数分裂中期Ⅰ制片的染色体进行原位杂交。结果表明,抗白粉病小麦品系GN22是普通小麦-簇毛麦二体代换系;用已定位在小麦第6部分同源群上的RFLP探针psr113、psr371进行Southern分析,进一步证明,小麦品系GN21、GN22是普通小麦-簇毛麦6A(6V)代换系;结合同工酶等电聚焦电泳分析,首次把簇毛麦编码的α-淀粉酶-1生化位点定位在簇毛麦6V染色体长臂上,暂命名为α-Amy-Ⅴ1。研究结果表明,原位杂交与RFLP技术相结合是全面、准确鉴定小麦外源染色体及其与小麦染色体部分同源关系的有效方法。
The total DNA of Haynaldia villosa genomic DNA was used as a probe to block the common wheat genomic DNA and to in situ hybridize with the chromosome of metaphase I of meiosis of pollen mother cells. The results showed that the resistant wheat powdery mildew strain GN22 was a common wheat-cluster-broomd two-body substitution line. The Southern analysis of psr113 and psr371 RFLP probes located on the homologue of wheat part 6 further demonstrated that wheat strain GN21 and GN22 were 6A (6V) substitution lines of common wheat-cluster hay, and the isozyme-based isoelectric focusing analysis showed that the biochemical sites of alpha-amylase-1 encoded by cluster hay Chromosome long arm, temporarily named α-Amy-Ⅴ1. The results show that the combination of in situ hybridization and RFLP technology is a comprehensive and accurate identification of wheat exogenous chromosomes and wheat chromosome homologous partial effective relationship.