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作者报道用常规福尔马林固定和石腊包埋的切片对人类胃癌浸润细胞核抗原(PCNA)进行免疫组织化学研究,并讨论其做为预后因素的意义。1974～1979年在日本Kyushu医院对138例原发胃癌进行了胃切除,其中92例为根治性切除。沿胃大弯或小弯切开,钉在木板上,用10%福尔马林固定。取中央组织标本作苏木精和嗜伊红染色,并取两个石腊块,作免疫组织化学染色以确定PCNA。所有染色的核定为PCNA阳性,观察切片1000核的最高标记次数,以确定PCNA标记指数,PCNA标记核的百分比用做分析。为了减少观察的偏差,另请一有经验的病理学家
The authors report the use of conventional formalin-fixed and paraffin-embedded sections to perform immunohistochemical studies on human gastric cancer infiltrating cell nuclear antigen (PCNA) and discuss its significance as a prognostic factor. From 1974 to 1979, gastrectomy was performed on 138 cases of primary gastric cancer at Kyushu Hospital in Japan, 92 of which were radical resections. Cut along a large or small curve of the stomach, nail it on a wooden board, and fix it with 10% formalin. The central tissue specimens were stained with hematoxylin and eosin, and two paraffin blocks were taken for immunohistochemical staining to determine PCNA. All stainings were verified as positive for PCNA, and the highest number of markings for the 1000 nuclear sections was observed to determine the PCNA labeling index. The percentage of PCNA labeled cores was used for analysis. In order to reduce the observation bias, please also ask an experienced pathologist