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根据GenBank已登陆的SRBSDV S10核苷酸序列设计引物,克隆SRBSDV江西分离物外壳蛋白(CP)基因,结合已公布的序列,通过生物信息学方法进行序列分析。结果表明,SRBSDV江西分离物CP基因全长1 674个核苷酸,推测编码557个氨基酸,与已报道的SRBSDV CP基因之间核苷酸同源性为97.5%～100%,氨基酸同源性为97.7%～100%。SRBSDV CP基因核苷酸序列高度保守,保守位点占全部位点的83.5%。没有发现重组事件。本研究首次报道根据系统发育进化树,SRBSDV可分为2大组群,不存在明显的地域和寄主分化。
Primers were designed according to the nucleotide sequence of SRBSDV S10 which landed in GenBank. The CP gene of SRBSDV Jiangxi isolate was cloned and sequenced by bioinformatics. The results showed that the CP gene of SRBSDV Jiangxi isolate was 1 674 nucleotides in length, which was deduced to encode 557 amino acids. The deduced amino acid sequence of the SRBSDV CP gene was 97.5% -100% homologous to the reported SRBSDV CP gene. 97.7% ~ 100%. The nucleotide sequence of SRBSDV CP gene was highly conserved, with conservative sites accounting for 83.5% of all loci. No reorganization event was found. According to phylogenetic tree, SRBSDV can be divided into two groups for the first time. There is no obvious geographical and host differentiation.