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[目的]探讨孕期暴露1800MHz电磁波对子代大鼠海马细胞凋亡的影响。[方法]以7周龄无特定病原体(SPF)级SD大鼠24只(雌鼠16只、雄鼠8只),雌雄2∶1合笼。孕鼠随机分为0.5mW/cm2暴露组、对照1组;1.0mW/cm2暴露组、对照2组;暴露组采用1800MHz射频电磁波对大鼠于孕第0~20天进行连续21d全身暴露,每天12h,功率密度分别为0.5 mW/cm2和1.0 mW/cm2。各组于出生后第3周和第7周时,分别随机抽取8只仔鼠(雌雄各半)测定其海马细胞凋亡率。[结果]孕期暴露于密度为1 800 MHz 0.5 mW/cm2的电磁波,3周龄仔鼠海马细胞凋亡率显著高于两对照组和1.0mW/cm2暴露组(P<0.05),1.0mW/cm2暴露组海马细胞凋亡率与两个对照组相比,差异无统计学意义(P>0.05);7周龄仔鼠海马细胞凋亡率,0.5mW/cm2和1.0mW/cm2组均高于两个对照组(P<0.05),两个对照组相比差异无统计学意义(P>0.05)。[结论]大鼠孕期暴露于1800MHz功率密度为0.5mW/cm2和1.0mW/cm2的电磁波可致发育期和成年期子代大鼠海马细胞凋亡率升高。
[Objective] To investigate the influence of 1800MHz electromagnetic wave during pregnancy on hippocampal apoptosis in offspring rats. [Method] Totally 24 Sprague - Dawley (SPF) SD rats of 7 weeks old (16 females and 8 males) were housed in male and female 2: 1 cohorts. Pregnant rats were randomly divided into 0.5mW / cm2 exposure group, control 1 group; 1.0mW / cm2 exposure group, control 2 groups; exposure group using 1800MHz radio frequency electromagnetic wave on rats in pregnancy 0 to 20 consecutive 21d systemic exposure every day 12h, the power density is 0.5 mW / cm2 and 1.0 mW / cm2 respectively. At the third week and the seventh week after birth, 8 offspring (male and female respectively) were randomly selected to determine the apoptosis rate of hippocampal cells in each group. [Results] The apoptosis rate in hippocampus of 3-week-old offspring was significantly higher than that of the two control groups and the exposure of 1.0mW / cm2 (P <0.05), the amplitude of 1.0mW / cm2 in hippocampus exposed group was significantly higher than that in the two control groups (P> 0.05); hippocampal apoptosis rate in hippocampus of 7-week-old mice was significantly higher than that of the control group (0.5mW / cm2 and 1.0mW / cm2) There was no significant difference between the two control groups (P <0.05) and the two control groups (P> 0.05). [Conclusion] The apoptosis rate of hippocampal cells of developing offspring rats and adult offspring of pregnant rats exposed to 1800MHz power density of 0.5mW / cm2 and 1.0mW / cm2 could be increased.