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应用免疫金标记抗体技术检测了沙田柚自花授粉后 1～ 3d花柱 1/2处通道细胞中识别蛋白定位分布 ,表明金颗粒依次定位于内质网 ,高尔基体、纤维壁 ,证实沙田柚花柱1/2处通道细胞中金颗粒分布的变化与花粉管在花柱中生长受阻位点和时间一致。 1/2花柱通道细胞纤维壁上的糖蛋白是沙田柚不亲和反应的识别位点。
The immunoprecipitation assay was used to detect the localization of recognition proteins in channel cells of passage 1 ~ 3 days after pollination in Shatian pomelo, indicating that the gold particles were located in endoplasmic reticulum, Golgi, and fiber wall in turn, The change of the distribution of gold particles in the channel of 1/2 channel was consistent with the blocked site and time of pollen tube growth in the style. Glycoprotein on the 1/2-style channel cell wall is the recognition site for the un-affinity reaction of Shatin pomelo.