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目的:观察PTEN、HER-2/neu和PKB在胆管癌组织中的表达,并探讨其与胆管癌发生、发展的关系及其意义.方法:应用免疫组织化学SP法检测80例胆管癌组织和24例正常胆管组织(取自其他良性疾病行胆管切除者)中PTEN、HER-2/neu和PKB的表达.结果:胆管癌组织中PTEN表达显著低于正常胆管组织(40.00%vs79.17%,χ2=11.33,P=0.001),HER-2/neu的表达显著高于正常胆管组织(73.75%vs33.33%,χ2=13.16,P=0.000),PKB的表达显著高于正常胆管组织(80.00%vs54.17%,χ2=6.41,P=0.01).PTEN和HER-2/neu蛋白表达呈明显负相关(r=-0.456,P=0.000);PTEN与PKB蛋白表达呈明显负相关(r=-0.355,P=0.001);HER-2/neu与PKB蛋白表达呈明显正相关(r=0.374,P=0.001).结论:PTEN表达的缺失和HER-2/neu过表达可能导致细胞增殖失控,与胆管癌的发生、发展密切相关,二者均与PKB呈相关性,提示二者均可能通过PI3K-PKB信号转导参与胆管癌的发生发展.
Objective: To observe the expression of PTEN, HER-2/neu and PKB in cholangiocarcinoma and to explore the relationship between PTEN, HER-2/neu and PKB and the occurrence and development of cholangiocarcinoma. Methods: To detect 80 cases of cholangiocarcinoma by immunohistochemical SP method Expression of PTEN, HER-2/neu, and PKB in 24 cases of normal bile duct tissue (biliary duct resection from other benign diseases). Results: PTEN expression in cholangiocarcinoma was significantly lower than normal bile duct tissue (40.00% vs 79.17% Χ2=11.33, P=0.001), HER-2/neu expression was significantly higher than normal bile duct tissue (73.75% vs 33.33%, χ2=13.16, P=0.000), and the expression of PKB was significantly higher than that of normal bile duct tissue ( 80.00% vs 54.17%, χ2=6.41, P=0.01). There was a significant negative correlation between PTEN and HER-2/neu protein expression (r=-0.456, P=0.000); PTEN was negatively correlated with PKB protein expression ( r=-0.355, P=0.001); HER-2/neu is positively correlated with PKB protein expression (r=0.374, P=0.001). Conclusions: Loss of PTEN expression and overexpression of HER-2/neu may result in cells Uncontrolled proliferation is closely related to the occurrence and development of cholangiocarcinoma. Both of them are related to PKB, suggesting that both may participate in the occurrence and development of cholangiocarcinoma through PI3K-PKB signal transduction.