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目的 观察短波紫外线（ＵＶＣ） 照射正常人外周血单个核细胞凋亡及白细胞介素２（ＩＬ－ ２） 、ＩＬ－ １２ 水平的影响。方法 采用５０ ｍＪ／ｃｍ２ ，２００ ｍＪ／ｃｍ２ 和４００ ｍＪ／ｃｍ２ 不同剂量的ＵＶＣ照射人体外周血单个核细胞，培养２４ 小时后用双抗体夹心ＥＬＩＳＡ 法检测细胞凋亡及ＩＬ－ ２ 、ＩＬ－ １２的含量。结果 ２００ ｍＪ／ｃｍ２ 、４００ ｍＪ／ｃｍ２ＵＶＣ 照射可明显诱发细胞凋亡（ｐｇ／ｍｌ） ，分别为４５ ．６８ ±１．３５，４６ ．６２ ±１．０２；照射后ＩＬ－ ２ 含量（ｐｇ／ｍｌ）明显下降（分别为１７６．８８±６ ．９７ ，１７４．１０ ±５ ．３６），与对照组（ｓＡＰＯ－ １／Ｆａｓ ４１．０９ ±１ ．６９ ，ＩＬ－ ２ 为１９２．９８ ±１１．９７ ｐｇ／ｍｌ） 比较差异均有非常显著性（ Ｐ＜０．０１） ，而ＩＬ－ １２ 含量无明显变化。结论 ＵＶＣ 照射可诱发人外周血单个核细胞凋亡，此外凋亡可能是ＵＶＣ引起的ＩＬ－ ２ 缺失所致。
Objective To observe the effects of ultraviolet (UV) radiation on the apoptosis of peripheral blood mononuclear cells and the levels of interleukin 2 (IL-2) and IL-12 in peripheral blood mononuclear cells. Methods Peripheral blood mononuclear cells were irradiated with different doses of UVC at doses of 50 mJ / cm 2, 200 mJ / cm 2 and 400 mJ / cm 2. After cultured for 24 hours, the cell apoptosis and IL-2, IL-12 Content. Results UVC irradiation at 200 mJ / cm2 and 400 mJ / cm2 significantly induced apoptosis (pg / ml), which was 45%. 68 ± 1.35,46. 62 ± 1.02. The levels of IL-2 (pg / ml) decreased significantly after irradiation (176.88 ± 6.97 and 174.10 ± 5.36, respectively) compared with the control group (sAPO- 1 / Fas 41). 09 ± 1 .69, 192.98 ± 11.97 pg / ml for IL-2) (P <0.01), while the content of IL-12 did not change significantly. Conclusion UVC can induce apoptosis in human peripheral blood mononuclear cells. In addition, apoptosis may be caused by UVC-induced IL-2 deficiency.