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目的:分析ELISA法检验乙肝标志物重要影响因素标本存放时间及存放条件对测定结果的影响。方法:对我院52例采用ELISA法检验乙肝标志物为阳性的样本,室温留样3天、5天和10天,2~6℃留样3天、5天和10天,分别再次采用ELISA法检测,分析标本存放时间及存放条件对检测结果的影响。结果:52例样本采用ELIsA法检测,当天样本乙肝标志物为阳性,室温存放3天后的样本为阳性,5天后有4例样本转变成阴性,10天后有11例样本转变成阴性。2~6℃存放3天、5天后样本均为阳性,10天后有8例样本转变为阴性。结论:采用ELISA法检验乙肝标志物,样本室温存放3天或2—6℃存放5天,对乙肝标志物检测结果无影响。
OBJECTIVE: To analyze the influence of storage time and storage conditions on the determination results of ELISA test for important influencing factors of hepatitis B markers. Methods: 52 cases in our hospital were tested by ELISA for samples with positive hepatitis B markers. Samples were stored for 3 days, 5 days and 10 days at room temperature, 3 days, 2 days and 6 days at 2 to 6 degrees for 3 days, 5 days and 10 days respectively. Law test, analysis of specimen storage time and storage conditions on the test results. Results: 52 samples were detected by ELIsA method. The samples of the day were positive for HBV markers. After 3 days of storage at room temperature, the samples were positive. After 5 days, 4 samples turned negative, and after 10 days, 11 samples turned negative. Samples were stored at 2 ~ 6 ℃ for 3 days after 5 days. After 10 days, 8 samples turned negative. Conclusion: Hepatitis B markers were detected by ELISA. The samples were stored at room temperature for 3 days or 2-6 ℃ for 5 days, and had no effect on the detection results of hepatitis B markers.