Detection of Interaction of Binding Affinity of Aromatic Hydrocarbon Receptor to the Specific DNA by

• 作 者: 孙晞 徐顺清
• 机 构: DepartmentofFoodScienceandEngineering,InstituteofEnvironmentalMedicine
• 出 处: 华中科技大学学报(医学英德文版)
• 发表日期: 2005年1期
• 关键词: 核酸外切酶 保护作用 检测方法 聚合酶链式反应 PCR DNA 芳香烃化合物 aryl hydrocarbon receptor dioxin-respons

A novel exonuclease protection mediated PCR assay (EPM-PCR) to detect the interaction of protein and DNA at a dioxin-responsive enhancer (DRE) upstream of the CYP1A1 gene in rat hepatic cytosol was established. A double-stranded DNA fragment containing tw