Enrichment,expansion,and identification of stem-like cells from hepatic carcinoma HepG2 cells

• 作 者: Biyu Cui Yebing Pang Xuehui Zh
• 机 构: ResearchDepartment
• 出 处: 广西医科大学学报
• 发表日期: 2017年1期
• 关键词: 广西医科大学 学报 hepatic carcinoma stem like cells enrich and culture
• 基金项目: Acknowledgments This work was supported by the National Natural Science Foundation of China （No. 81360347, No. 81560493）, Key Project of Natural Science Foundation of Guangxi （No. 2015GXNSFDA 139024）

Objective:To establish a method for enriching,culturing and identifying stem-like subpopulation from human hepatic carcinoma cells,and to explore its biological properties.Methods:HepG2cells were cultured in cancer stem cell(CSC)medium to form spheroids.T