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为了研究染色体畸变与微核形成的关系,本实验用不同浓度的丝裂霉素C(MMC,0.025—0.4μg/ml),处理人外周血淋巴细胞,观察中期染色体畸变与不同细胞周期形成的微核间的关系。获得如下主要结果:(1)MMC诱发的染色体畸变细胞率(ACF),未经培养的G_0期淋巴细胞的微核细胞率(NC-MNCF)以及培养的淋巴细胞的微核细胞率(C-MNCF),在一定剂量范围内均呈剂量依赖性增加,并可用幂回归方程描述;(2)微核形成与染色体畸变全然无关的NC-MNCF,和C-MNCF一样,与ACF呈良好的正相关;(3)用胞质分裂阻滞(CB)法,检测MMC诱发的CB-MNCF,较C-MNCF无显著提高,MNCF/ACF的比值较小,并随着MMC剂量增加从0.15左右降到0.03。所有上述结果表明,不能简单理解微核形成与染色体畸变间的关系,在分裂的细胞群体中,中期染色体畸变可能仅是微核形成的一种来源。
In order to study the relationship between chromosome aberration and micronucleus formation, we treated human peripheral blood lymphocytes with different concentrations of mitomycin C (MMC, 0.025-0.4μg / ml) to observe the relationship between metaphase chromosome aberration and different cell cycle formation Relationship between micronuclei. The main results were as follows: (1) The rate of chromosomal aberrations induced by MMC (ACF), the number of micronucleated cells (NC-MNCF) in non-cultured G 0 lymphocytes and the percentage of micronucleated cells in cultured lymphocytes (C- MNCF) in a dose-dependent manner increased in a dose-dependent manner and described by power regression equation. (2) NC-MNCF with micronucleus formation unrelated to chromosomal aberrations, like C-MNCF, showed good positive correlation with ACF (3) CB-MNCF induced by MMC was detected by cytoplasmic cleavage block (CB) method. Compared with C-MNCF, CB-MNCF was not significantly increased, and the ratio of MNCF / ACF was smaller. To 0.03. All of the above results suggest that the relationship between micronuclei formation and chromosomal aberrations can not be easily understood and that in the split cell population, metaphase chromosome aberrations may be only one source of micronucleus formation.