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目的:观察天香丹对载脂蛋白E基因敲除[Apo E(-/-)]小鼠血清中白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)表达的影响,探讨天香丹防治动脉粥样硬化(AS)的可能机制。方法:48只8周龄Apo E(-/-)小鼠分为正常对照组(12只,给予普通饲料室温饲养)和造模组(36只,高脂饲料复合气候箱干预)。喂养12周后,成功复制Apo E(-/-)小鼠AS秽浊痰阻证模型后,分为3组:模型组、天香丹组、阿托伐他汀组,每组10只。用药12周后处死各组小鼠,采集标本,HE染色,光镜下观察主动脉病理学变化,并测定斑块面积(PA)与管腔面积(LA)之比;ELISA法检测血清中IL-1β和TNF-α含量。结果:与正常对照组比较,模型组主动脉PA/LA比值上升(P<0.01),血清中IL-1β和TNF-α表达水平均升高(P<0.01);与模型组比较,天香丹组、阿托伐他汀组PA/LA比值均减小(P<0.01),血清中IL-1β和TNF-α表达水平均降低(P<0.01)。结论:天香丹具有防治AS的作用,其机制可能与其降低炎性因子IL-1β和TNF-α的表达,抑制炎性反应有关。
AIM: To observe the effect of Tianxiangdan on the expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in serum of apolipoprotein E gene knockout [Apo E To explore the possible mechanism of Tianxiangdan in preventing and treating atherosclerosis (AS). Methods: A total of 48 Apo E (- / -) mice of 8 weeks old were divided into normal control group (12 rats fed normal feed at room temperature) and model group (36 rats fed with high fat diet). After 12 weeks of feeding, AS obstructing phlegm blocking model of Apo E (- / -) mice was successfully divided into 3 groups: model group, Tianxiangdan group and atorvastatin group, with 10 rats in each group. After 12 weeks, the mice in each group were sacrificed, and the specimens were collected and stained with HE for histopathological changes. The ratio of plaque area (PA) to lumen area (LA) was determined by light microscopy. IL -1β and TNF-α content. Results: Compared with the normal control group, the PA / LA ratio in the aorta increased (P <0.01) and the levels of IL-1β and TNF-α in the model group increased (P <0.01) (P <0.01), and the levels of IL-1β and TNF-α in serum decreased (P <0.01). CONCLUSION: Tianxiangdan can prevent and treat AS. Its mechanism may be related to its decreasing the expression of inflammatory factors IL-1β and TNF-α and inhibiting the inflammatory reaction.