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目的对皮孔葱臭木Dysoxylum lenticellatum茎干的化学成分及其细胞毒活性进行研究。方法利用正相硅胶、RP-18柱色谱、葡聚糖凝胶Sephadex LH-20等手段进行分离纯化,根据波谱数据鉴定化合物的结构,采用MTT法进行细胞毒活性测定。结果分离鉴定了12个化合物,分别为β-谷甾醇(1)、豆甾醇(2)、3-oxo-24,25,26,27-tetranortirucall-7-ene-23(21)-lactone(3)、3α-hydroxy-24,25,26,27-tetranortirucall-7-ene-23(21)-lactone(4)、laxiracemosin H(5)、laxiracemosin B(6)、24,25-epoxy-tirucall-7-ene-3,23-dione(7)、丁酰鲸鱼醇(8)、齐墩果酸(9)、蒲公英赛酮(10)、蒲公英赛醇(11)和8-hydroxy-6-methoxy-3-pentylisocoumarin(12)。结论 12个化合物均为首次从该种植物中分离得到,化合物8、10～12为首次从该属植物中分离得到,化合物5、6是甘遂烷型生物碱,化合物3具有明显体外肿瘤细胞生长抑制活性。
Objective To study the chemical constituents and cytotoxic activity of Dysoxylum lenticellatum stem. Methods The compounds were separated and purified by normal phase silica gel, RP-18 column chromatography and Sephadex LH-20 dextran gel. The structures of the compounds were identified by spectral data. The cytotoxicity was determined by MTT assay. Results Twelve compounds were isolated and identified as beta-sitosterol (1), stigmasterol (2), 3-oxo-24,25,26,27-tetranortirucall-7-ene-23 ), 3α-hydroxy-24,25,26,27-tetranortirucall-7-ene-23 (21) -lactone (4), laxiracemosin H (5), laxiracemosin B (6), 24,25-epoxy- tirucall- 7-ene-3,23-dione (7), butylated hydroxytoluene (8), oleanolic acid (9), dandelion alone (10), dandelion alcohol -3-pentylisocoumarin (12). CONCLUSION Twelve compounds were isolated from this plant for the first time. Compounds 8 and 10 to 12 were isolated from this genus for the first time. Compounds 5 and 6 were akanethirane-type alkaloids and compound 3 had significant in vitro tumor cells Growth inhibitory activity.