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目 的:探究新生仔猪口服鼠李糖乳杆菌GG对肠道屏障功能的影响.创新点:新生仔猪早期口服鼠李糖乳杆菌GG可明显改善其断奶前肠道菌群结构及免疫屏障功能.方法:二十四头新生仔猪分为对照组和实验组:对照组仔猪在出生后第1、3、5天口服2mL0.1 g/mL的脱脂牛奶;而实验组仔猪口服等体积的含有活鼠李糖乳杆菌GG的脱脂牛奶.饲喂25天后,收集仔猪血清、肠道粘膜和盲肠内容物等样品.通过分析肠道菌群、紧密连接蛋白和细胞因子等指标,评价鼠李糖乳杆菌对肠道屏障功能的影响.结论:在正常生理条件下,新生仔猪口服鼠李糖乳杆菌GG可明显改变肠道菌群结构.此外,鼠李糖乳杆菌GG还可增加仔猪肠道的通透性,并通过调控抗菌肽、细胞因子和趋化因子的分泌以改善肠道的免疫屏障功能.“,”To understand the effects of Lactobacillus rhamnosus GG (ATCC 53103) on intestinal barrier function in pre-weaning piglets under normal conditions,twenty-four newborn littermate piglets were randomly divided into two groups.Piglets in the control group were orally administered with 2 mL 0.1 g/mL sterilized skim milk while the treatment group was administered the same volume of sterilized skim milk with the addition of viable L.rhamnosus at the 1st,3rd,and 5th days after birth.The feeding trial was conducted for 25 d.Results showed that piglets in the L.rhamnosus group exhibited increased weaning weight and average daily weight gain,whereas diarrhea incidence was decreased.The bacterial abundance and composition of cecal contents,especially Firmicutes,Bacteroidetes,and Fusobacteria,were altered by probiotic treatment.In addition,L.rhamnosus increased the jejunal permeability and promoted the immunologic barrier through regulating antimicrobial peptides,cytokines,and chemokines via Toll-like receptors.Our findings indicate that oral administration of L.rhamnosus GG to newborn piglets is beneficial for intestinal health of pre-weaning piglets by improving the biological,physical,and immunologic barriers of intestinal mucosa.