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目的 探讨抗核周因子 (APF)对类风湿关节炎 (RA)的诊断价值及其与RA病情的相关。方法 以健康人颊粘膜细胞为底物 ,在不同血清稀释度下采用间接免疫荧光法检测 37例RA患者、36例其它风湿病患者及5例正常人血清APF ,同时评估RA病情活动性。结果 在 1∶2 0血清稀释度下 ,APF对RA诊断的敏感性为73%,特异性为 97 6 %。活动期与非活动期RA的APF阳性率存在差异 ,但无统计学意义。结论 APF可作为RA的诊断指标 ,但可能与RA的病情活动无关。 1∶2 0为最佳待检血清稀释度。
Objective To investigate the diagnostic value of anti-perinuclear factor (APF) on rheumatoid arthritis (RA) and its relationship with RA. Methods Serum APF in 37 patients with RA, 36 other rheumatic diseases and 5 normal controls were detected by indirect immunofluorescence at different serum dilutions in healthy human buccal mucosa cells. Meanwhile, the activity of RA was evaluated. Results At 1: 200 serum dilution, APF had a sensitivity of 73% and a specificity of 97.6% for RA. Active and inactive RA APF positive rate differences, but not statistically significant. Conclusion APF can be used as a diagnostic indicator of RA, but may not be related to the disease activity of RA. 1:20 for the best sera to be tested dilution.