论文部分内容阅读
目的:建立一种简便的体外扩增小鼠树突状细胞(DCs)的方法。方法:联合应用粒细胞-巨噬细胞集落刺激因子(GM-CSF)、白细胞介素-4(IL-4)诱导培养小鼠骨髓单个核细胞分化为DCs,并从形态学、表型及功能方面对其加以检测。结果:体外诱导培养8 d后获得大量成熟的DCs,DCs表面具有典型的树枝状突起,并表达髓系DCs特异性表面分子CD11c(83.19%)、CD86(89.24%)及MHC-Ⅱ(95.25%),同时具有很强的刺激同种异基因混合淋巴细胞增殖的能力。结论:小鼠骨髓细胞体外诱导培养可生成大量功能成熟的DCs,为进一步抗肿瘤疫苗的研究奠定了基础。
Objective: To establish a simple and convenient method for the expansion of mouse dendritic cells (DCs) in vitro. Methods: Bone marrow mononuclear cells were induced to differentiate into DCs induced by GM-CSF and IL-4. The morphological, phenotypic and functional Its detection. RESULTS: A large number of mature DCs were obtained after cultured for 8 days in vitro. DCs showed typical dendrites and expressed CD11c (83.19%), CD86 (89.24%) and MHC-Ⅱ (95.25% ), While having a strong ability to stimulate allogeneic mixed lymphocyte proliferation. CONCLUSION: Mice bone marrow cells can induce a large number of mature DCs induced by in vitro culture, which lays the foundation for the further study of antitumor vaccine.