论文部分内容阅读
应用单克隆抗体免疫组织化学方法,显示胚胎及生后大鼠肾脏的γ-谷氨酰转肽酶(γ-GTP)。从胎龄第18天起,γ-GTP在近端小管细胞刷状缘、侧面及基底面膜上反应即呈阳性,生后1～3天反应呈强阳性。随着肾单位的发育,免疫反应虽有所减弱,但生后第30天仍可看到中等强度的反应。其中近端小管S_3段的免疫反应最强,皮质深层的近端小管比皮质浅层的反应强。细段降支的立方或扁平细胞的腔面及侧面细胞膜上,免疫反应亦呈阳性。免疫电镜观察表明,生后1～7天,在近端小管上皮细胞顶部微绒毛、胞膜内陷、吞饮小泡及侧面和基底面细胞膜上,免疫反应均呈阳性,但紧密连接处反应为阴性。基底部质膜内褶的发生及γ-GTP定位,似与周围毛细血管的发生密切相关。此外,γ-GTP还存在于肾间质细胞膜上,并偶见于细胞质内。
Monoclonal antibody immunohistochemistry was used to show γ-glutamyl transpeptidase (γ-GTP) in the kidney of embryonic and postnatal rats. From the 18th day of gestational age, γ-GTP reacts positively with the brush border, lateral and basal lamina of proximal tubule cells. The reaction is strongly positive 1 to 3 days after birth. With the development of the nephron, although the immune response weakened, but 30 days after birth can still see moderate-intensity response. The proximal tubule S_3 segment of the strongest immune response, cortical deep proximal tubules than the cortical shallow reaction. Fine section of the stem or cubic cell flat surface and lateral cell membrane, the immune reaction also showed positive. Immune electron microscopy showed that 1 to 7 days after birth in the proximal tubule epithelial cells at the top of the microvilli, membrane collapse, swallowing small vesicles and lateral and basal cell membrane, the immune response was positive, but tight junction reaction Negative. The occurrence of basal plasma membrane folds and γ-GTP localization may be closely related to the occurrence of peripheral capillaries. In addition, γ-GTP also exists in the renal interstitial membrane, and occasionally in the cytoplasm.