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自从PCR产生以来，已经为增大PCR产物的长度做过很大的努力。每一种新的PCR产物都必须被鉴定，测序仍是最重要鉴定方法。克服了最初的困难之后，PCR产物的直接测序做为一种首选方法建立起来而克隆的PCR产物的测序，因为没有校正耐热多聚酶错误掺入的功能而往往不被选用。较长的
Since PCR production, great efforts have been made to increase the length of PCR products. Each new PCR product must be identified and sequencing is still the most important method of identification. After overcoming the initial difficulties, direct sequencing of PCR products was sequenced as a preferred method of cloning PCR products because they were not often used without correcting the misincorporation function of heat-resistant polymerases. longer