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目的研究赖氨酰氧化酶(LOX)在吸烟与非吸烟患者非小细胞肺癌组织、癌旁组织、正常肺组织中的表达,探讨LOX与非小细胞肺癌发生发展的关系,吸烟与LOX的关系,吸烟与非小细胞肺癌的关系。方法实时荧光逆转录聚合酶链式反应(RT-PCR)检测44例非小细胞肺癌组织,癌旁组织,正常肺组织中LOX mRNA含量;Western Blot检测44非小细胞肺癌组织,癌旁组织,正常肺组织中LOX蛋白表达水平。并进行统计学分析。结果所检测组织标本中LOX mRNA及LOX蛋白的表达率均呈阶梯式表现,即非小细胞肺癌组织>癌旁组织>正常肺组织,表达差异有统计学意义(P<0.05)。吸烟组LOX mRNA和LOX蛋白的表达率低于非吸烟组,差异有统计学意义(P<0.05)。结论 44例非小细胞肺癌组织中LOX均呈过度表达状态,LOX可能与非小细胞肺癌的发生发展有关。而吸烟的非小细胞肺癌组织中LOX表达低于非吸烟非小细胞肺癌组织。吸烟可能通过抑制LOX的表达从而影响非小细胞肺癌的发生与发展。
Objective To investigate the expression of lysyl oxidase (LOX) in non-small cell lung cancer tissues, paracancerous tissues and normal lung tissues of smokers and non-smokers and to explore the relationship between the LOX and the development of non-small cell lung cancer and the relationship between smoking and LOX , Smoking and non-small cell lung cancer. Methods The mRNA levels of LOX in 44 non-small cell lung cancer tissues, paracancerous tissues and normal lung tissues were detected by real-time fluorescence reverse transcription polymerase chain reaction (RT-PCR). Western Blot was used to detect the expression of LOX mRNA in 44 non-small cell lung cancer tissues, LOX protein expression level in normal lung tissue. And statistical analysis. Results The expression rates of LOX mRNA and LOX protein in the tissue samples showed a stepwise expression. The expression of LOX mRNA and LOX protein in the non-small cell lung cancer tissues was significantly higher than that in the paracancerous tissues> normal lung tissues (P <0.05). The expression of LOX mRNA and LOX protein in smoking group was lower than that in non-smoking group, the difference was statistically significant (P <0.05). Conclusion LOX in 44 NSCLC tissues is overexpressed, and LOX may be related to the occurrence and development of non-small cell lung cancer. However, LOX expression in smoking non-small cell lung cancer tissues was lower than that in non-smoking non-small cell lung cancer tissues. Smoking may affect the occurrence and development of non-small cell lung cancer by inhibiting the expression of LOX.