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Objective:To investigate the in vitro effects of the antihacterial drugs,mcropenem trihydrate.piperacillin sodium,and cefoperazone sodium,on the activity of human serum paraoxonase mPOND.Methods:hPQN1 was purified from human serum using simple chromatographic methods.including DEAE-Sephadex anion exchange and Sephadex G-200 gel filtration chromatography.Results:The three antihacterial drugs decreased in vitro hPON1 activity.Inhibition mechanisms meropcnem trihydrate was noncompetitive while piperacillin sodium and cefoperazone sodium were competitive.Conclusions:Our results showed that antihacterial drugs significantly inhibit hPON1 activity,both in vitro,with rank order meropenem trihydrate piperacillin sodium cefoperazone sodium in vitro.
Objective: To investigate the in vitro effects of the antihacterial drugs, mcropenem trihydrate. Piperacillin sodium, and cefoperazone sodium, on the activity of human serum paraoxonase mPOND. Methods: hPQN1 was purified from human serum using simple chromatographic methods. INC DEAE-Sephadex anion Exchange and Sephadex G-200 gel filtration chromatography. Results: The three antihacterial drugs decreased in vitro hPON1 activity. Inhibitory meropcnem trihydrate was noncompetitive while piperacillin sodium and cefoperazone sodium were competitive. Confclusions: Our results showed that antihacterial drugs significantly inhibit hPON1 activity, both in vitro, with rank order meropenem trihydrate piperacillin sodium cefoperazone sodium in vitro.