论文部分内容阅读
目的:描述波兰耐药结核分枝杆菌分离株的特征,估计人群中近期传播的数量。设计:应用间隔区寡核苷酸分型及 IS6110DNA 指纹图谱法对2000年波兰251名耐药肺结核患者分离株进行分析。对部分菌株进行 rpoB、katG 和/或 inhA基因调节区测序分析。结果:应用间隔区寡核苷酸分型/IS6110-RFLP 相结合的成簇定义,29%的菌株成簇,提示存在近期传播。成簇菌株中某些病例的传播联系得到了流行病学资料证实,多数分离株的突变与利福平和异烟肼耐药相关。年龄、性别、移民身份、用药史和成簇没有关系,耐多药病例更容易成簇。在波兰,也发现了北京基因型分离株,但频率较邻国偏低。结论:证实了耐药结核菌的传播及其对波兰发生耐药结核病的作用。
Objective: To characterize the resistant M. tuberculosis isolates in Poland and estimate the number of recent transmission in the population. DESIGN: An analysis of 251 isolates from poorer polio patients in Poland in 2000 was performed using spacer oligonucleotide typing and IS6110 DNA fingerprinting. Partial strains were sequenced for rpoB, katG and / or inhA gene regulatory regions. RESULTS: Using clustered definitions of spacer oligonucleotide typing / IS6110-RFLP, 29% of the strains were clustered, suggesting the recent spread. Epidemiological data confirm the transmission of some cases in clusters of strains, and mutations in most isolates are associated with rifampicin and isoniazid resistance. Age, gender, immigration status, medication history and clustering have no relationship, and MDR-susceptible cases are more likely to cluster. In Poland, Beijing genotypes were also found, but at lower frequencies than their neighbors. Conclusion: Confirmed the spread of drug-resistant TB and its role in developing drug-resistant TB in Poland.