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离脊髓70mm处挟伤大鼠右侧坐骨神经,左、右腹角注入~3H亮氨酸,液闪测定腹根、坐骨、腓总及胫神经中标记蛋白的放射活性以确定它在轴浆中的转运距离。无论挟伤神经后立即注射~3H亮氨酸,1—98天后测定(慢速转运,SAT),或挟伤后0—98天后注射,6h后测定(快速转运,FAT),伤后2天内标记蛋白的轴浆转运均阻断在损伤点附近。3天后转运功能重新出现,7天的转运距离仍明显小于对照[对照:91.7±1.7mm(FAT);93.7±1.7mm(SAT)]。14—98天,标记蛋白的转运距离均与对照相似(P>0.05)。将伤后14天
The right sciatic nerve of the right sciatic nerve was implanted at a distance of 70 mm from the spinal cord, and 3H-leucine was injected into the left and right ventral horn of the spinal cord. The radioactivity of the labeled protein in the ventral root, ischial, common peroneal, and tibial nerve was determined by liquid scintillation to confirm it in the axoplasm Transit distance. 3H-leucine was injected immediately after nociceptive nerves, measured 1-98 days later (slow transit, SAT), or 0-98 days post wounding, measured 6 hours later (rapid transit, FAT), within 2 days after injury Axonal plasma transport of the tagged proteins was blocked near the injury site. After 3 days, the transport function reappeared, and the transport distance in 7 days was still significantly smaller than that in the control [control: 91.7 ± 1.7 mm (FAT); 93.7 ± 1.7 mm (SAT)]. At 14-98 days, the transport distances of the marker proteins were similar to those of the control (P> 0.05). It will be 14 days after injury