高效液相色谱法测定人体中依普拉芬血药浓度

• 作 者: 曾珍 王自正 马晓红 刘天培
• 机 构: 南京市第一医院,南京医科大学临床药理研究所
• 出 处: 中国医院药学杂志
• 发表日期: 1997年05期
• 关键词: 拉芬 血药浓度 药物动力学 高效液相色谱法 生物利用度 药动学 口服吸收 检测限 分析柱 二室模型

以反相高效液相色谱法测定血清中依普拉芬的浓度，血清样品用乙腈直接提取。色谱条件为：分析柱使用ＳｐｈｅｒｉｓｏｒｂＣ１８柱（１０μｍ），４．６×１５０ｍｍ；流动相甲醇∶水＝７１∶２９（Ｖ／Ｖ）；检测波长为ＵＶ２４５ｎｍ；本法的最低检测限为３ｎｇ／ｍｌ，线性范围８～４００ｎｇ／ｍｌ，回收率为８４．７％。并对８名受试者口服４００ｍｇ依普拉芬后的药物动力学与生物利用度进行了研究，结果表明：依普拉芬为口服吸收二室模型，主要药动学参数为Ｔ１／２９．０～１２．０ｈ，Ｔｍａｘ２．４～２．５ｈ，Ｃｍａｘ分别为６６．５～６６．８ｎｇ／ｍｌ。国产依普拉芬相对于进口依普拉芬的生物利用度为１００．２％。

The concentration of epiprofen in serum was determined by reversed-phase high-performance liquid chromatography, and the serum samples were directly extracted with acetonitrile. The chromatographic conditions were as follows: Spherisorb C18 column (10μm), 4.6 × 150mm; mobile phase methanol: water = 71:29 (V / V); detection wavelength was UV245nm; the detection limit of this method was 3ng / Linear range of 8 ~ 400ng / ml, recovery was 84.7%. The pharmacokinetics and bioavailability of epigalafen after oral administration of 400 mg of 8 subjects were studied. The results showed that Iprafen was the two-compartment oral absorption model with the main pharmacokinetic parameters being T1 / 29. 0 ~ 12.0h, Tmax2.4 ~ 2.5h, Cmax respectively 66.5 ~ 66.8ng / ml. The bioavailability of domestic Iprafen relative to imported Iprafen was 100.2%.