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黄瓜第 2叶喷施 75mmol/LK2 HPO4 溶液以后 ,每天采取叶片 ,用去离子水、1mol/L氯化钠、2 %醋酸和 5%盐酸 4种浸提液连续提取不同形态的钙 ,其中水溶性钙和果胶酸钙的含量减少而磷酸钙含量明显上升 ,同时 ,叶片中相关的抗病酶活性也持续升高。用焦锑酸钙沉淀的电镜细胞化学方法研究了叶片内Ca2 + 定位分布的变化后发现 ,与对照比较 ,喷施K2 HPO4 处理 ,液泡和叶绿体出现大量的钙颗粒分布 ,同时细胞间层中的钙沉淀也变得更为密集。用X 射线能谱仪测得细胞壁上Ca/P明显下降。上述现象表明 ,叶片细胞液泡和叶绿体含钙量增加以及磷酸钙沉淀的形成使得中间层的钙桥键断裂 ,从而引起抗病酶的产生 ,并导致细胞壁分解形成寡聚半乳糖醛酸 ,可诱导黄瓜苗产生系统抗病能力
After the second leaf of cucumber was sprayed with 75mmol / L K2HPO4 solution, leaves were taken daily and different forms of calcium were continuously extracted with four kinds of extracts of deionized water, 1mol / L sodium chloride, 2% acetic acid and 5% hydrochloric acid, Calcium and calcium pectinate decreased while the content of calcium phosphate increased significantly. At the same time, the activity of anti-disease enzymes in the leaves also continued to increase. The change of Ca2 + localization in leaves was studied by electron microscopy cytochemistry with calcium pyroantimonate precipitation. It was found that when K2 HPO4 was sprayed, a large number of calcium particles were distributed in the vacuoles and chloroplasts compared with the control. At the same time, Calcium precipitation also becomes more dense. X-ray energy dispersive spectroscopy measured cell wall Ca / P decreased significantly. The above phenomenon indicates that the increase of calcium content in leaf vacuoles and chloroplasts and the formation of calcium phosphate precipitate break the calcium bridges in the middle layer and cause the generation of resistance enzymes and lead to the decomposition of the cell wall to form oligogalacturonic acid which can be induced Cucumber seedlings produce systemic disease resistance