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目的:用单细胞凝胶电泳技术(SCGE)研究急慢性缺氧大鼠海马DG区神经细胞细胞DNA损伤和人参皂甙对缺氧大鼠海马细胞DNA的保护作用。方法:健康成年SD大鼠随机分为急、慢性缺氧正常对照组、急性缺氧组和慢性缺氧组(分别在模拟海拔5000米高原环境连续缺氧暴露0d、3d和30d)、急性缺氧人参皂甙干预组、慢性缺氧人参皂甙干预组,应用SCGE检测海马DG区神经细胞DNA损伤。结果:随着缺氧时间的增加,海马DG区神经细胞DNA的损伤程度加重,尾长、尾部DNA百分含量和尾距显著增加(P<0.05)。人参皂甙能使缺氧损伤的海马DG区神经细胞的尾长、尾部DNA百分含量和尾距均较缺氧组减少(P<0.05)。结论:人参皂甙能有效地减轻缺氧引起的海马组织细胞DNA的断裂损伤。
OBJECTIVE: To study DNA damage of hippocampal DG cells and ginsenoside on the DNA damage of hippocampus in acute hypoxic rats by single cell gel electrophoresis (SCGE). Methods: Healthy adult Sprague-Dawley rats were randomly divided into acute and chronic hypoxia control group, acute hypoxia group and chronic hypoxia group (0d, 3d and 30d after continuous hypoxia exposure simulated altitude of 5,000 m) Oxygen ginsenoside intervention group and chronic hypoxia ginsenoside intervention group, DNA damage of DG in hippocampus was detected by SCGE. Results: With the increase of hypoxia time, the damage of DNA in hippocampus DG neurons increased, the tail length, tail DNA content and tail length increased significantly (P <0.05). Ginsenoside can make the tail length, tail DNA content and tail length of hippocampal DG neurons decreased compared with those in hypoxia group (P <0.05). Conclusion: Ginsenosides can effectively reduce DNA damage in hippocampal cells induced by hypoxia.