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已有的研究结果表明,肝素可以作为β2-整合素(Mac-1)的配体抑制炎症过程中Mac-1介导的嗜中性粒细胞与血管内皮细胞的黏附.通过选择性化学修饰方法制备了具有低抗凝血活性的高碘酸氧化-硼氢化钠还原肝素(RO-肝素),系统地研究了它对Mac-1介导的嗜中性粒细胞黏附的抑制作用.结果表明,显著失去抗凝血活性的RO-肝素仍能有效地抑制Mac-1介导的嗜中性粒细胞与ICAM-1重组蛋白、转染ICAM-1 cDNA的COS-7细胞和人脐静脉内皮细胞黏附.为深入阐明拮抗Mac-1介导的白细胞黏附的分子机制和筛选抗炎症药物提供了有价值的实验证据.
Previous studies have shown that heparin can inhibit Mac-1-mediated neutrophil adhesion to vascular endothelial cells as a ligand for β2-integrin (Mac-1) during inflammatory processes. By selective chemical modification A periodic acid oxidation-sodium borohydride reduction heparin (RO-heparin) with low anticoagulant activity was prepared and its inhibitory effect on Mac-1-mediated neutrophil adhesion was systematically investigated. The results showed that, RO-heparin, which has a significant loss of anticoagulant activity, is still effective in inhibiting Mac-1-mediated neutrophil-ICAM-1 recombinant protein, COS-7 cells transfected with ICAM-1 cDNA and human umbilical vein endothelial cells Adhesion and provide valuable experimental evidence for further elucidating the molecular mechanism of antagonizing Mac-1-mediated leukocyte adhesion and screening anti-inflammatory drugs.