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目的探索氢醌(hydroquinone,HQ)致DNA整体低甲基化的后续结果。方法用磷酸盐缓冲液(PBS)溶解HQ,以PBS处理组为对照组,分别以2.5、5.0、10.0和20.0μmol/L HQ染毒TK6细胞为处理组。应用实时荧光定量-聚合酶链反应检测白血病相关原癌基因MPL、BRAF、ERBB2、MYB、MYC和RAF1的表达水平。结果与对照细胞相比,HQ处理细胞MPL和RAF1的mRNA表达量都随HQ剂量增加而上升,其中20μmol/L HQ对原癌基因表达的影响最为明显,分别上升了80%(P<0.05)和35%(P<0.05);MYB、MYC和ERBB2基因的mRNA表达量随HQ的剂量上升而下降。结论 MPL的转录活化很有可能与HQ导致的DNA整体低甲基化有关。
Objective To explore the subsequent results of DNA hypomethylation induced by hydroquinone (HQ). Methods HQ was dissolved in phosphate buffered saline (PBS) and treated with PBS as the control group. TK6 cells were treated with 2.5, 5.0, 10.0 and 20.0 μmol / L HQ, respectively. The expression of leukemia proto-oncogene MPL, BRAF, ERBB2, MYB, MYC and RAF1 were detected by real-time fluorescence quantitative polymerase chain reaction. Results Compared with the control cells, the mRNA expression levels of MPL and RAF1 in HQ-treated cells increased with the increase of HQ dose. The effect of 20 μmol / L HQ on the expression of proto-oncogene was the most significant, up by 80% (P <0.05) And 35% respectively (P <0.05). The mRNA expression of MYB, MYC and ERBB2 decreased with the increase of HQ dose. Conclusion The transcriptional activation of MPL is likely to be associated with HQ-induced overall DNA hypomethylation.