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目的研究茶树油皮肤消毒液反复使用对哺乳动物的毒性,为茶树油消毒剂的进一步开发利用提供理论依据。方法按照《消毒技术规范》(2002年版),采用大、小鼠急性经口毒性试验、小鼠骨髓嗜多染红细胞微核试验、家兔皮肤刺激性毒性试验和大鼠亚急性毒性试验,观察茶树油皮肤消毒液对实验动物的毒性作用。结果大、小鼠的急性经口毒性试验结果显示,茶树油皮肤消毒液的LD50均大于5 000mg/kg BW,属实际无毒级;小鼠骨髓嗜多染红细胞微核试验结果为阴性,未发现该受试样品对体细胞有诱变作用;新西兰家兔的多次完整皮肤刺激实验结果,每只动物的平均积分小于0.5,属无刺激性;亚急性毒性试验,给药量在1 000mg/kg BW以下,各剂量组与阴性对照组比较,大鼠的体重、脏体系数、血清生化指标及血常规无显著影响,脏器组织病理学检查未发现异常。结论在本实验条件下,茶树油皮肤消毒液毒性较低,刺激性小,将其开发成消毒剂具有广阔的前景。
Objective To study the toxicity of tea tree oil skin disinfectant to mammals repeatedly and provide a theoretical basis for the further development and utilization of tea tree oil disinfectant. Methods According to the “Disinfection Technical Specification” (2002 edition), the acute oral toxicity test in mice and mice, the micronucleus test in mouse bone marrow polychromatic erythrocytes, the skin irritant toxicity test in rabbits and the subacute toxicity test in rats were observed Toxicity of tea tree oil skin disinfectant to experimental animals. Results The results of acute oral toxicity test showed that the LD50 of tea tree oil skin disinfectant was more than 5000 mg / kg BW, which was actually non-toxic. The micronucleus test results of bone marrow polychromatic erythrocytes in mice were negative, Found that the test sample has a mutagenic effect on somatic cells; New Zealand rabbit multiple complete skin irritation test results, the average integral of each animal is less than 0.5, is non-irritating; subacute toxicity test, the dose of 1 000mg / kg BW or less, each dose group compared with the negative control group, rat body weight, dirty body coefficient, serum biochemical indicators and blood no significant effect, histopathological examination showed no abnormalities. Conclusions Under the experimental conditions, the skin disinfectant of tea tree oil has low toxicity and little irritation, which has broad prospects for developing into a disinfectant.