L-NNA对门脉高压大鼠离体血管反应性的影响

• 作 者: 吴金生 李席如 何泽生 马庆久 高德明
• 机 构: 第四军医大学唐都医院普外科
• 出 处: 新消化病学杂志
• 发表日期: 1997年06期
• 关键词: 门脉高压 L-NNA 血管反应性 高血压.门脉 肌.平滑.血管 疾病模型.动物 精氨酸 部分门静脉结扎 大鼠离体 收缩反应

目的探讨门脉高压症血管反应性变化与一氧化氮（ＮＯ）的关系．方法采用部分门静脉结扎大鼠为模型，研究加和不加一氧化氮合成酶抑制剂Ｎω硝基Ｌ精氨酸（ＮωｎｉｔｒｏＬａｒｇｉｎｉｎｅ，ＬＮＮＡ）两种情况下，离体腹主动脉条对不同浓度的氯化钾（ＫＣｌ）（１０ｍｍｏｌ／Ｌ～８０ｍｍｏｌ／Ｌ）和苯肾上腺素（１０９ｍｏｌ／Ｌ～１０４ｍｏｌ／Ｌ）的收缩反应性变化．结果ＫＣｌ的ＥＣ５０值（２６５ｍｍｏｌ／Ｌ±０９ｍｍｏｌ／Ｌ）明显高于对照组（２３３ｍｍｏｌ／Ｌ±１７ｍｍｏｌ／Ｌ，Ｐ＜００１），苯肾上腺素的ＥＣ５０值（３７２ｎｍｏｌ／Ｌ±０４ｎｍｏｌ／Ｌ）也显著高于对照组（２８６ｎｍｏｌ／Ｌ±０２ｎｍｏｌ／Ｌ，Ｐ＜００１）．显示部分门静脉结扎组大鼠腹主动脉对ＫＣｌ和苯肾上腺素的收缩反应下降．应用ＬＮＮＡ后，门脉高压组和对照组ＫＣｌ的ＥＣ５０值分别为２１１８ｍｍｏｌ／Ｌ±０８ｍｍｏｌ／Ｌ和１９４ｍｍｏｌ／Ｌ±１２ｍｍｏｌ／Ｌ，二者相差不显著（Ｐ＞００５）；ＫＣｌ的最大发展张力（１０８ｇ±０１ｇ）仍低于对照组（１２１ｇ±０１１ｇ，Ｐ＜００５）．苯肾上腺素的ＥＣ５０值（２２４?

Objective To investigate the relationship between vascular reactivity and nitric oxide (NO) in patients with portal hypertension. Methods Part of the portal vein ligation rats as a model to study the addition and non-nitric oxide synthase inhibitor Nω  nitro  L  arginine (Nω  nitro  Larginine, L  NNA) two cases, The contractile response of isolated abdominal aortic strips to different concentrations of potassium chloride (KCl) (10 mmol / L ~ 80 mmol / L) and phenylephrine (10-9 mol / L ~ 10 -4 mol / L) Results The EC50 values ​​of KCl (265 mmol / L ± 09 mmol / L) were significantly higher than those of the control group (233 mmol / L ± 17 mmol / L, P <001) 37.2nmol / L ± 0.4nmol / L) was also significantly higher than the control group (28.6nmol / L ± 0.2nmol / L, P <0.01). Shrinkage of KCl and phenylephrine in the abdominal aorta of rats with portal vein ligation was decreased. After application of LNNA, the EC50 values ​​of KCl in portal hypertension group and control group were 2118mmol / L ± 08mmol / L and 194mmol / L ± 12mmol / L, respectively, with no significant difference (P > 005). The maximum developmental tension of KCl (108g ± 01g) was still lower than that of the control group (121g ± 011g, P <005). Phenylephrine EC50 value (22  4?