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目的:探讨山莨菪碱对急性肝损伤的保护作用及其作用机制.方法:雄性WISTAR大鼠18只随机分为3组:正常对照组、模型组和山莨菪碱组.以D半乳糖胺加内毒素脂多糖制成大鼠急性肝损伤模型.用山莨菪碱(20MG/KG)预处理模型鼠.观察3组大鼠肝酶含量,血清一氧化氮(NO)含量,肝组织中NO、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性及肝组织病理学变化.结果:山莨菪碱组大鼠丙氨酸转氨酶〔ALT(666±267)U〕、天冬氨酸转氨酶〔AST(1059±408)U〕及血清总胆红素〔TBIL(7.22±5.62)μMOL/L〕均明显低于模型组〔分别为(2352±698)U,(3549±717)U和(20.71±5.92)μMOL/L〕,P均＜0.01;伴随着山莨菪碱对血及肝组织中NO过量合成的抑制,肝组织中MDA降低、SOD增高(P均＜0.01).山莨菪碱组肝组织病理改变较模型组明显减轻.结论:山莨菪碱对大鼠急性肝损伤有保护作用,保护作用可能与山莨菪碱抑制NO的过量产生及清除自由基有关.“,”Objective:To study the protective effect of anisodamine on acute liver injury and its potential mechanism.Methods:Rats were injected intraperitoneally with DGalactosamine (DGalN, 0 6 g/kg ) plus lipopolysaccharide (LPS,0 1 mg/kg) to induce acute liver injury.Animals were randomly divided into normal control group,liver injury group and treatment group (pretreatment with anisodamine 20 mg/kg).Twelve hours later,serum and liver tissues were collected to measure liver function parameters,lipid peroxidation...