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作者用霍乱弧菌稻叶型569B株免疫小鼠,取其脾细胞与骨髓瘤细胞Sp2/0-Ag14融合,获得产生抗体的杂交瘤细胞17株。对所产生的抗霍乱肠毒素(cT)及其亚单位(CT一A、cT一a)的单克隆抗体(McAb)用多种免疫学方法分析其特性,以大肠杆菌
The authors used Vibrio cholerae 569B strain of mice to immunize mice and spleen cells were fused with myeloma cells Sp2 / 0-Ag14 to obtain antibody-producing hybridoma cells 17. Monoclonal antibodies (McAbs) against the produced cholera enterotoxin (cT) and its subunits (CT-A, cT-a) were analyzed for their characteristics by a variety of immunological methods. Escherichia coli